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Updated: Jun 9, 2026

Development and Application of Rapamycin-regulated Tyrosine Phosphatases
Published on: September 6, 2024
Targeting the reversibly oxidized protein tyrosine phosphatase superfamily
Benoit Boivin1, Ming Yang, Nicholas K Tonks
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Abstract:
Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
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