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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jun 9, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Plant protein-protein interaction network and interactome.

Yixiang Zhang1, Peng Gao, Joshua S Yuan

  • 1Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA.

Current Genomics
|September 3, 2010
PubMed
Summary

Understanding plant protein-protein interactions is key to systems biology. This review explores plant interactome mapping methods and their applications in plant development and stress responses.

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Related Experiment Videos

Last Updated: Jun 9, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

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Published on: March 3, 2015

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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

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mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

Area of Science:

  • Plant biology
  • Systems biology
  • Molecular biology

Background:

  • Protein-protein interaction networks are crucial for understanding biological processes.
  • The plant interactome provides insights into plant development, physiology, and pathology.

Purpose of the Study:

  • To define the plant interactome and protein-protein interaction networks.
  • To discuss the significance of plant interactome studies.
  • To compare various interactome mapping strategies.

Main Methods:

  • Yeast two-hybrid system (Y2H)
  • Affinity purification mass spectrometry (AP-MS)
  • Bimolecular fluorescence complementation (BiFC)
  • In silico prediction

Main Results:

  • Comparison of pros and cons for different interactome mapping techniques.
  • Discussion of platform applications in specific plant biology questions.
  • Highlighting the potential of plant interactome studies in elucidating molecular mechanisms.

Conclusions:

  • Plant interactome mapping is essential for understanding signal transduction, stress responses, and development.
  • Advancements in interactome mapping reveal great potential for future plant biology research.
  • Mapping the plant interactome in model species will guide future studies.