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Related Experiment Video

Updated: Jun 9, 2026

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines

Published on: March 22, 2015

Mesenchymal stem cells promote mammosphere formation and decrease E-cadherin in normal and malignant breast cells.

Ann H Klopp¹, Lara Lacerda, Anshul Gupta

¹Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.

|September 3, 2010

Summary

This summary is machine-generated.

Mesenchymal stem cells (MSC) enhance breast cancer stem cell mammosphere formation and promote tumor growth. MSCs also decrease E-cadherin, a marker linked to breast cancer progression and therapy resistance.

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Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Published on: November 8, 2014

Area of Science:

Oncology
Stem Cell Biology
Biomedical Research

Background:

Normal and malignant breast tissues harbor rare, self-renewing stem cells or tumor-initiating cells (TIC).
These cells can be enriched through mammosphere culture in 3D.

Purpose of the Study:

To investigate if human bone-marrow derived mesenchymal stem cells (MSC) enhance mammosphere formation.
To determine the impact of MSCs on breast cancer stem cell properties and tumor development.

Main Methods:

Assessing mammosphere formation in response to MSCs and MSC-conditioned media.
Analyzing cell adhesion molecule expression (E-cadherin, N-cadherin).
Evaluating tumor development in vivo after co-injection with MSCs.

Main Results:

MSCs dose-dependently increased mammosphere formation in HMEC, MCF-7, and SUM149 cells.
MSC-conditioned media significantly boosted mammosphere formation (6.4-21 fold).
Co-injection with MSCs reduced tumor latency and increased tumor growth, with decreased E-cadherin expression.

Conclusions:

MSCs enhance primary mammosphere formation in normal and malignant breast cells.
MSCs decrease E-cadherin expression, a factor associated with breast cancer progression and treatment resistance.