Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A systematic review of human evidence for the intergenerational effects of exposure to ionizing radiation.

International journal of radiation biology·2024
Same author

M-FISH evaluation of chromosome aberrations to examine for historical exposure to ionising radiation due to participation at British nuclear test sites.

Journal of radiological protection : official journal of the Society for Radiological Protection·2024
Same author

No evidence of increased mutations in the germline of a group of British nuclear test veterans.

Scientific reports·2022
Same author

British nuclear test veteran family trios for the study of genetic risk.

Journal of radiological protection : official journal of the Society for Radiological Protection·2022
Same author

Higher Incidence of Chromosomal Aberrations in Operators Performing a Large Volume of Endovascular Procedures.

Circulation·2022
Same author

Dose estimation after a mixed field exposure: Radium-223 and intensity modulated radiotherapy.

Nuclear medicine and biology·2021

Related Experiment Video

Updated: Jun 9, 2026

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
06:26

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

Multiplex fluorescence in situ hybridization (M-FISH).

Rhona Anderson1

  • 1Laboratory of Genome Damage, Division of Biosciences, Centre for Cell and Chromosome Biology, Brunel University, Uxbridge, Middlesex, UK. Rhona.anderson@brunel.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2010
PubMed
Summary

Multiplex in situ hybridization (M-FISH) is a 24-color karyotyping technique for analyzing complex chromosome rearrangements. This method enables precise identification of structural and numerical abnormalities through combinatorial fluorescent labeling and digital image analysis.

More Related Videos

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
15:54

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Published on: June 14, 2014

Related Experiment Videos

Last Updated: Jun 9, 2026

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
06:26

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
15:54

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Published on: June 14, 2014

Area of Science:

  • Cytogenetics
  • Molecular Biology
  • Genomics

Background:

  • Complex interchromosomal rearrangements pose challenges for traditional karyotyping.
  • Multiplex in situ hybridization (M-FISH) offers a comprehensive solution for detailed chromosomal analysis.

Purpose of the Study:

  • To describe the methodology and applications of 24-color M-FISH.
  • To highlight M-FISH as a key technique for resolving complex chromosomal abnormalities.

Main Methods:

  • Combinatorial labeling of all human chromosomes with spectrally distinct fluorophores.
  • Microscopic visualization and digital image acquisition using specialized filters and software.
  • Superimposition of fluorophore images for chromosome classification based on combinatorial labeling.

Main Results:

  • Unique spectral signatures generated for each homologous chromosome pair.
  • Accurate classification of chromosomes based on fluorophore composition.
  • Detailed analysis of digital images to identify structural and numerical abnormalities.

Conclusions:

  • M-FISH is an effective 24-color karyotyping technique.
  • It is the preferred method for studying complex interchromosomal rearrangements.
  • M-FISH facilitates precise resolution of chromosomal abnormalities.