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Related Experiment Video

Updated: Jun 9, 2026

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

PNA-FISH on human sperm.

Franck Pellestor¹, Cécile Monzo, Samir Hamamah

¹Service de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, CHRU Montpellier, Montpellier, France. f-pellestor@chu-montpellier.fr

Methods in Molecular Biology (Clifton, N.J.)

|September 3, 2010

Summary

Peptide nucleic acids (PNAs) offer a fast and specific method for identifying human chromosomes. This technique is now adapted for assessing aneuploidy in male gametes using PNA-FISH.

Area of Science:

Biochemistry
Genetics
Cytogenetics

Background:

Peptide nucleic acids (PNAs) are synthetic nucleic acid analogs with a peptide-like backbone.
PNAs exhibit high affinity and specificity for complementary DNA and RNA sequences.
PNA applications have expanded into cytogenetics for chromosome identification.

Purpose of the Study:

To describe the adaptation of multicolor PNA-FISH protocols for human spermatozoa.
To establish a rapid procedure for assessing aneuploidy in male gametes.

Main Methods:

Utilized multicolor PNA-FISH protocols.
Adapted these protocols for application to human spermatozoa.
Focused on rapid in situ identification of human chromosomes.

More Related Videos

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Published on: September 1, 2009

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Last Updated: Jun 9, 2026

Fish Sperm Assessment Using Software and Cooling Devices

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Published on: September 1, 2009

Main Results:

Demonstrated the efficacy of PNA-FISH in cytogenetics.
Successfully adapted PNA-FISH for human spermatozoa analysis.
Developed a new, fast procedure for aneuploidy assessment in male gametes.

Conclusions:

PNA-based methods provide efficient chromosome identification.
PNA-FISH is a valuable tool for assessing male gamete aneuploidy.
This technique offers a significant advancement in reproductive genetics analysis.