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Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...

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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

A scanning frequency mode for ion cyclotron mobility spectrometry.

Rebecca S Glaskin1, Stephen J Valentine, David E Clemmer

  • 1Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.

Analytical Chemistry
|September 3, 2010
PubMed
Summary

This study introduces a novel operational mode for ion cyclotron mobility spectrometry, achieving high-resolution separations by using resonant oscillating fields. This method effectively separates peptide ions, demonstrating tunable resolving power for advanced analytical applications.

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectrometry

Background:

  • Ion mobility spectrometry (IMS) is a powerful analytical technique for separating ions based on their size and shape.
  • Achieving high-resolution separations in IMS often requires complex instrumentation or lengthy analysis times.

Purpose of the Study:

  • To explore a new operational mode for ion cyclotron mobility spectrometry (IC-MS) to enhance separation resolution.
  • To demonstrate the capability of this new mode for separating complex peptide mixtures.

Main Methods:

  • Utilized oscillating electric fields applied to segmented regions of a circular drift tube in an IC-MS instrument.
  • Ions were separated based on their resonant mobilities with the applied field frequency.
  • Analyzed mixtures of substance P peptide and tryptic digests of cytochrome c.

Main Results:

  • Successfully separated different charge states and conformers of substance P peptide ions ([M+2H](2+) and [M+3H](3+)).
  • Achieved resolving powers exceeding 300, with a maximum of ~400 at high cycle numbers.
  • Demonstrated that resolving power is tunable by adjusting the ion cycle number.

Conclusions:

  • The novel operational mode offers a promising approach for high-resolution ion mobility separations.
  • The ability to tune resolving power provides significant analytical flexibility for complex mixture analysis.
  • This technique has substantial potential for applications in proteomics and other fields requiring precise ion separation.