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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Updated: Jun 9, 2026

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

On solid-phase antibody assays.

Octavio E Pajaro¹, James F George

¹Division of Cardiothoracic Surgery, University of Alabama at Birmingham, 1530 3rd Avenue S., Birmingham, AL, USA. opajaro@uab.edu

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation

|September 3, 2010

Summary

This summary is machine-generated.

Detecting anti-human leukocyte antigen (anti-HLA) antibodies is crucial in thoracic transplantation. New solid-phase assays raise concerns, highlighting the need for functional tests to understand antibody significance in rejection.

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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

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Published on: December 14, 2016

Area of Science:

Immunology and Transplantation Science
Cardiovascular and Thoracic Surgery

Background:

Pre- and post-operative anti-human leukocyte antigen (anti-HLA) antibodies are recognized as detrimental in thoracic transplantation.
The role, diagnosis, and treatment of antibody-mediated rejection (AMR) remain subjects of ongoing discussion in heart and lung transplantation.

Purpose of the Study:

To address concerns regarding new technologies for anti-HLA antibody detection in transplantation.
To emphasize the necessity of functional assays for interpreting the clinical significance of anti-HLA antibodies.

Main Methods:

Review of current evidence and technological advancements in anti-HLA antibody detection.
Discussion of solid-phase assays and their implications in thoracic transplantation.

Main Results:

New solid-phase assay technologies for anti-HLA antibody detection present challenges and require careful consideration.
Existing detection methods may not fully capture the clinical relevance of antibody-mediated rejection.

Conclusions:

Accurate detection of anti-HLA antibodies is central to managing AMR in thoracic transplantation.
Functional assays are essential to complement solid-phase assays, providing deeper clinical insights into antibody-mediated rejection.