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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 9, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

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Published on: October 5, 2017

Rapid microsatellite development for water striders by next-generation sequencing.

Jen C Perry¹, Locke Rowe

¹Department of Ecology and Evolutionary Biology, University of Toronto, Toronto ON M5S 3B2, Canada. jen.perry@utoronto.ca

The Journal of Heredity

|September 3, 2010

Summary

This summary is machine-generated.

Researchers developed new genetic markers for water striders, crucial for studying sexual conflict and coevolution. This breakthrough uses next-generation sequencing to create valuable molecular tools for ecological research.

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Last Updated: Jun 9, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

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Published on: October 5, 2017

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

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Published on: November 5, 2020

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Published on: April 17, 2015

Area of Science:

Ecology
Genetics
Evolutionary Biology

Background:

Water striders are key models for studying sexual conflict and coevolution.
Limited genetic resources hinder research progress in these model organisms.
Next-generation sequencing offers a cost-effective way to develop molecular markers for non-model organisms.

Purpose of the Study:

To develop novel microsatellite markers for the water strider Gerris incognitus.
To facilitate future research on sexual selection, population genetics, and coevolution in water striders.

Main Methods:

Genomic DNA from Gerris incognitus was sequenced using Roche 454 technology.
Microsatellite loci were identified from sequencing reads.
Primers were designed and tested for amplification and polymorphism in G. incognitus and Gerris buenoi.

Main Results:

Sequencing yielded 182,912 reads, with 16.8% containing microsatellite repeats.
10 polymorphic microsatellite loci were identified, showing an average of 6.1 alleles per locus.
These markers proved effective in both G. incognitus and the related species G. buenoi.

Conclusions:

Light sequencing of genomic DNA is an effective method for developing numerous, highly variable microsatellite markers.
These new genetic resources will advance studies on sexual conflict, population structure, and phylogeography in water striders.