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Updated: Jun 9, 2026

Experimental Metastasis Assay

Experimental Metastasis Assay

Published on: August 24, 2010

Experimental metastasis assay.

Sonali Mohanty¹, Lei Xu

¹Department of Biomedical Genetics, University of Rochester, USA.

Journal of Visualized Experiments : Jove

|September 3, 2010

Summary

This summary is machine-generated.

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This study details an experimental metastasis assay using immunodeficient mice to understand cancer spread. The assay helps identify key factors driving metastatic progression in human cancer cells.

Area of Science:

Oncology
Cancer Biology
Immunology

Background:

Metastasis is the primary cause of cancer-related mortality.
Understanding the mechanisms of metastasis is crucial for developing effective cancer therapies.

Purpose of the Study:

To describe the establishment and procedures of an experimental metastasis assay.
To utilize this assay for investigating the metastatic potential of human cancer cells.
To generate highly metastatic cell lines for further molecular studies.

Main Methods:

An experimental metastasis assay was developed using immunodeficient mice.
Human cancer cells were prepared in vitro and injected intravenously into mice.
Metastases in organs, particularly the lungs, were quantified.

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Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

Lung Tumor Cell Recruitment Assay

Lung Tumor Cell Recruitment Assay

Published on: February 26, 2019

Related Experiment Videos

Last Updated: Jun 9, 2026

Experimental Metastasis Assay

Experimental Metastasis Assay

Published on: August 24, 2010

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

Lung Tumor Cell Recruitment Assay

Lung Tumor Cell Recruitment Assay

Published on: February 26, 2019

Metastatic lesions were isolated and cultured to establish new cell lines.

Main Results:

The assay allows for the quantification of metastatic ability correlated with injected cancer cell numbers.
Cultured metastases often exhibit enhanced metastatic potential compared to parental cell lines.
This method generates valuable tools for studying metastatic progression.

Conclusions:

The experimental metastasis assay is a robust model for studying cancer metastasis.
The generated highly metastatic cell lines are instrumental in identifying genes and pathways regulating metastasis.
This research provides a foundation for further investigations into cancer cell dissemination and therapeutic targets.