Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autosomal allelic inactivation at loci with variable replication timing and dosage sensitivity.

eLife·2026
Same author

Transcription elongation can be sufficient, but is not necessary, to advance replication timing.

EMBO reports·2026
Same author

Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks.

Molecular cell·2026
Same author

Microhomology-mediated end joining acts directly on replication forks to repair single-ended double strand breaks.

bioRxiv : the preprint server for biology·2026
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same author

Autosomal Allelic Inactivation: Variable Replication and Dosage Sensitivity.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 9, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Evaluating genome-scale approaches to eukaryotic DNA replication.

David M Gilbert1

  • 1Department of Biological Science, Florida State University, Tallahassee, 32306, USA. gilbert@bio.fsu.edu

Nature Reviews. Genetics
|September 3, 2010
PubMed
Summary
This summary is machine-generated.

Understanding eukaryotic DNA replication origins is challenging. Genome-scale methods are advancing our knowledge of replication timing and initiation sites across eukaryotes, despite data gaps in multicellular organisms.

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Related Experiment Videos

Last Updated: Jun 9, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • The precise mechanisms governing eukaryotic DNA replication initiation remain largely unknown.
  • While replication origin identification is established in yeasts, data for multicellular organisms are limited.
  • Replication timing evaluation methods have yielded extensive data across eukaryotic systems.

Purpose of the Study:

  • To review genome-scale methodologies for analyzing eukaryotic DNA replication.
  • To discuss the types of research questions addressable by these methods.
  • To highlight technical challenges in fully characterizing eukaryotic replication origins.

Main Methods:

  • Genome-scale methods for DNA replication analysis.
  • Techniques for identifying replication origins and associated proteins.
  • Protocols for evaluating replication timing across the genome.

Main Results:

  • Genome-scale approaches offer powerful tools for studying DNA replication.
  • Replication timing data is widely available for many eukaryotic systems.
  • Significant challenges persist in comprehensively identifying and understanding replication origins.

Conclusions:

  • Genome-scale methods are crucial for dissecting eukaryotic DNA replication.
  • Further methodological development is needed to overcome current limitations.
  • A complete understanding of eukaryotic replication origins requires integrated approaches.