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Updated: Jun 9, 2026
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Development of chemiluminescence method for determination of 10-hydroxycamptothecin based on luminol-[Ag(HIO₆)₂]⁵⁻
Hanwen Sun1, Peiyun Chen, Shasha Shi
1College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Wusi East Road 180, Baoding 071002, China.
Abstract:
A novel chemiluminescence (CL) method was developed for the determination of 10-hydroxycamptothecin(HCPT) based on the CL reaction between [Ag(HIO₆)₂]⁵⁻ and luminol in alkaline solution. CL emission of Ag(III) complex-luminol in alkaline medium was very different from that in acidic medium. A possible mechanism of enhanced CL emission was suggested. The enhanced effect of HCPT on CL emission of the [Ag(HIO₆)₂]⁵⁻-luminol system was found. The enhanced degree of CL emission was proportional to HCPT concentration. The effect of the reaction conditions on CL emission was examined. Under optimal conditions, the limit of detection was 6.5 × 10⁻⁹ g mL⁻¹. The proposed method was applied for the determination of HCPT in real samples with the recoveries of 93.2-109% with the RSD of 1.7-3.3%.
