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Genetic manipulation of Mycobacterium abscessus

Halima Medjahed1, Anil Kumar Singh

  • 1Université Paris Descartes, Faculté de Médecine Paris Descartes, Paris, France.

Current Protocols in Microbiology
|September 3, 2010
PubMed

Abstract:

This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.

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