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Cytomegalovirus Disease

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...

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Human cytomegalovirus: propagation, quantification, and storage.

William J Britt¹

¹University of Alabama School of Medicine, Birmingham, Alabama, USA.

Current Protocols in Microbiology

|September 3, 2010

Summary

Human cytomegalovirus (HCMV) propagation and quantification present challenges due to its large size and long replication cycle. This study provides protocols for laboratory strains, aiding researchers in HCMV research.

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Area of Science:

Virology
Molecular Biology
Cell Biology

Background:

Human cytomegalovirus (HCMV) is a complex human herpesvirus, specifically a beta-herpesvirus.
HCMV exhibits species specificity, replicating only in human cells, primarily primary and some transformed cell lines.
The virus has a prolonged replicative cycle of approximately 48 hours, posing propagation and quantification challenges.

Purpose of the Study:

To describe protocols for the propagation of laboratory strains of human cytomegalovirus (HCMV).
To detail methods for the accurate quantification of HCMV.
To outline procedures for the concentration and gradient purification of HCMV for downstream applications.

Main Methods:

Standard cell culture techniques for viral propagation.
Methods for viral particle quantification (e.g., plaque assays, qPCR).
Ultracentrifugation and gradient purification techniques for virus concentration.

Main Results:

Established protocols for consistent propagation of laboratory HCMV strains.
Validated methods for reliable HCMV quantification.
Optimized procedures for high-purity HCMV concentration and purification.

Conclusions:

The provided protocols address technical challenges in HCMV handling.
These methods facilitate reproducible HCMV research and downstream applications.
Standardized protocols enhance the study of HCMV biology and pathogenesis.