Laboratory growth and maintenance of Haemophilus influenzae

Haemophilus influenzae is a non-spore forming, non-motile, pleomorphic Gram-negative coccobacilli. H. influenzae is a strict human pathogen that can cause a variety of systemic and localized infections, depending on the strain. The three Basic Protocols in this unit will describe how to culture and prepare H. influenzae for the use in biological studies of the organism.