Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acetylcholinesterase from Wistar rat brain.

C Vidal, J A Lozano, A Soler

    Revista Espanola De Fisiologia
    |March 1, 1978
    PubMed
    Summary

    Toluene storage alters acetylcholinesterase properties, affecting enzyme activity measurements. Freshly extracted enzyme shows consistent properties, indicating toluene

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Decalogue of the Declaration of Salamanca: Advancing Undergraduate Education in Allergy and Clinical Immunology Across Europe.

    Allergy·2026
    Same author

    Outcomes of Kaposi sarcoma in people living with HIV who maintain long-term viral suppression: A prospective case-series.

    HIV medicine·2026
    Same author

    Anthropogenically-induced saline wetland recharge drives denitrification: Implications for nitrate attenuation in semi-arid aquifer-wetland systems.

    Journal of contaminant hydrology·2026
    Same author

    A pharmacist-led educational and audit & feedback strategy to reduce antibiotic prescription in primary care: the AFA study.

    BMC primary care·2025
    Same author

    Ongoing multiparameter unrest at the Montagne Pelée volcano on Martinique from 2019 to 2024.

    Scientific reports·2025
    Same author

    Code Anaphylaxis: Enhancing the Management of Hymenoptera Venom Anaphylaxis.

    Journal of investigational allergology & clinical immunology·2025

    Area of Science:

    • Biochemistry
    • Enzymology
    • Neuroscience

    Background:

    • Acetylcholinesterase (AChE) is a crucial enzyme in neurotransmission.
    • Enzyme properties can be influenced by storage and extraction methods.
    • Understanding AChE behavior is vital for neurological research.

    Purpose of the Study:

    • To investigate the effects of toluene storage on rat brain acetylcholinesterase.
    • To characterize the properties of partially purified AChE.
    • To explore the impact of different assay methods on enzyme activity.

    Main Methods:

    • Partial purification of acetylcholinesterase from toluene-stored rat brains.
    • Extraction using non-ionic detergents and buffers.
    • Activity assays using short-time radiometric and long-time colorimetric methods.
    • pH optimum determination, thermal denaturation studies, and substrate concentration effects.
    • Gel filtration and disc electrophoresis for enzyme form analysis.

    Main Results:

    • Toluene storage significantly altered AChE properties compared to fresh brain extracts.
    • Two pH optima (7.5-8.0 and 8.0-8.6) were observed for enzyme activity.
    • Assay method influenced results: high substrate activated with short-time assay, inhibited with long-time assay.
    • Evidence suggests a catalytic role for a histidine residue.
    • Gel filtration and electrophoresis revealed multiple, interconvertible AChE forms.

    Conclusions:

    • Toluene treatment introduces artifacts affecting acetylcholinesterase characterization.
    • The choice of assay method critically impacts observed enzyme kinetics and substrate inhibition.
    • Rat brain acetylcholinesterase exists in multiple forms, potentially interconvertible.

    Related Experiment Videos