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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Microtubule Formation01:23

Microtubule Formation

Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation of...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.

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Related Experiment Video

Updated: Jun 9, 2026

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Nucleating the assembly of macromolecular complexes.

Kimberly J Peterson-Kaufman1, Clayton D Carlson, José A Rodríguez-Martínez

  • 1Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.

Chembiochem : a European Journal of Chemical Biology
|September 3, 2010
PubMed
Summary

Researchers are designing synthetic molecules to control cellular processes by mimicking nature's complex interactions. These molecules can nucleate new biological interactions, offering precise control over cellular machinery for various applications.

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Last Updated: Jun 9, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Chemistry

Background:

  • Nature utilizes intricate molecular complexes for cellular process regulation.
  • Researchers aim to engineer synthetic molecules to mimic and control these natural interactions.

Purpose of the Study:

  • To develop synthetic molecules capable of nucleating natural and unnatural interactions.
  • To enable precise modulation of cellular pathways through engineered molecular complexes.

Main Methods:

  • Design and synthesis of novel molecular compounds.
  • Application of synthetic molecules to direct protein-protein, protein-lipid, protein-DNA, and protein-RNA interactions.
  • Recruitment or replacement of natural cellular components with synthetic agents.

Main Results:

  • Demonstrated ability of synthetic molecules to drive protein dimerization.
  • Successful modulation of interactions between various biomolecules (proteins, lipids, DNA, RNA).
  • Engineered multicomponent complexes with synthetic molecules offer enhanced control over cellular machinery.

Conclusions:

  • Synthetic molecules provide a powerful tool for controlling cellular processes.
  • Engineered molecular interactions can be leveraged for diverse applications.
  • This approach offers unprecedented control over cellular machinery by directing complex formation.