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Updated: Jun 9, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Investigation of the experimental limits of small-sample heteronuclear 2D NMR
Bruce D Hilton1, Gary E Martin
1Discovery and Preclinical Sciences, Process Chemistry Rapid Structure Characterization Laboratory, Merck Research Laboratories, Summit, New Jersey 07901, USA.
Abstract:
Practical experimental performance limits for an ensemble of heteronuclear 2D NMR experiments using a state-of-the-art 600 MHz 1.7 mm Bruker TCI Micro CryoProbe are reported. In the specific case of multiplicity-edited GHSQC, it was possible to acquire data on a 540 ng sample of strychnine (1; ∼1.6 nmol), prepared by serial dilution, which was used as a model compound. The experiments discussed also included GCOSY, (13)C reference spectra, (1)H-(13)C GHMBC, IDR-GHSQC-TOCSY, 1,1-ADEQUATE, and (1)H-(15)N GHMBC.
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