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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Instrumentation00:57

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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A novel approach to modelling counter-current chromatography.

Hacer Guzlek1, Ines I R Baptista, Philip L Wood

  • 1Imperial College London, South Kensington Campus, London SW7 2AZ, UK. Hguzlek@imperial.ac.uk

Journal of Chromatography. A
|September 4, 2010
PubMed
Summary

A new counter-current chromatography (CCC) model predicts chromatograms using column dimensions and experimental settings, eliminating the need for empirical calibration. This robust model aids in accurate retention time, peak width, and resolution prediction for separation optimization.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Counter-current chromatography (CCC) models exist for predicting solute retention and peak width.
  • Previous models often rely on empirical calibration values.

Purpose of the Study:

  • To develop a novel CCC model that predicts chromatograms from fundamental parameters.
  • To validate the model's accuracy and robustness across various CCC instruments and experimental conditions.

Main Methods:

  • Developed a CCC model linking parameters directly to column dimensions and experimental settings.
  • Validated the model using experimental data from diverse CCC instruments.
  • Assessed the impact of mobile phase flow rate, rotational speed, and β-value on model predictions.

Main Results:

  • The model accurately predicts solute retention time, peak width, and peak resolution.
  • Model predictions showed reasonable accuracy across a range of CCC instruments.
  • The model demonstrated robustness and applicability to various experimental parameters.

Conclusions:

  • The developed CCC model offers a powerful tool for parameter estimation without empirical calibration.
  • This approach significantly enhances separation optimization in counter-current chromatography.