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Updated: Jun 9, 2026
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
Published on: March 22, 2011
[Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells]
Geng-zhen Chen1, Hui Hu, Ming-yan Xu
1Third Department of Surgery, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, China. chengengzhen@tom.com
Objective:
To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721.
Methods:
The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays.
Results:
The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells.
Conclusion:
A replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.
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