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Updated: Jun 9, 2026
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Polymerase chain reaction confirmation of diagnosis of intestinal amebiasis in Puducherry
Subhash Chandra Parija1, Atul Garg, K Pushpa
1Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry 605006, India. subhashparija@yahoo.co.in
Background:
Stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate Entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic Entamoeba dispar and Entamoeba moshkovskii.
Methods:
Two hundred and forty-six stool samples collected between January to February 2009, were examined microscopically for E. histolytica/E. dispar/E. moshkovskii complex cysts and trophozoites. Nested multiplex PCR targeting a 16 S-like rRNA gene for differential detection of all the three morphologically similar forms of E. histolytica, E. moshkovskii and E. dispar simultaneously was done on all microscopy positive stool samples.
Results:
Forty-nine stool samples were positive for Entamoeba spp. by microscopy. Nested multiplex PCR for E. histolytica, E. moshkovskii and E. dispar was positive in only 19 of these samples, and only 6 of the 19 samples were positive for E. histolytica.
Conclusion:
Stool microscopy grossly over-diagnosed intestinal amebiasis and is likely to result in unnecessary treatment with anti-protozoal drugs.
