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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 9, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Isolation of tissue mast cells.

Marianna Kulka1, Dean D Metcalfe

  • 1National Research Council Canada, Institute for Nutrisciences and Health, Charlottetown, Prince Edward, Island, Canada.

Current Protocols in Immunology
|September 4, 2010
PubMed
Summary

Researchers developed a new protocol to isolate viable and enriched mast cells from human tissues. This method improves upon traditional techniques, aiding allergy and inflammation research.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Mast cells are key effector cells in allergic inflammation, differentiating in tissues and exhibiting heterogeneity.
  • Current isolation methods for human tissue mast cells often yield low viability and purity.

Purpose of the Study:

  • To describe a novel protocol for isolating viable and enriched human tissue mast cells.
  • To improve upon existing methods for mast cell isolation from tissues like lung and skin.

Main Methods:

  • Gentle enzymatic digestion of tissues.
  • Positive selection techniques for cell enrichment.
  • Combined approach to enhance cell viability and purity.

Main Results:

  • Successful isolation of reasonably viable mast cell preparations.

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Isolation of Functional Cardiac Immune Cells
07:26

Isolation of Functional Cardiac Immune Cells

Published on: December 5, 2011

Related Experiment Videos

Last Updated: Jun 9, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Isolation of Functional Cardiac Immune Cells
07:26

Isolation of Functional Cardiac Immune Cells

Published on: December 5, 2011

  • Substantially enriched populations of tissue-derived mast cells obtained.
  • Improved yield and quality compared to traditional methods.

    • Conclusions:

    • The described protocol offers a more effective means of obtaining high-quality mast cells for research.
    • This method facilitates further investigation into mast cell heterogeneity and function in allergic inflammation.