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Updated: Jun 9, 2026
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019
[Detection and sequences analysis of sheep hepatitis E virus RNA in Xinjiang autonomous region]
1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Objective:
To understand whether hepatitis E virus (HEV) was infectious in sheep in Xinjiang.
Methods:
We used reverse transcription-nested polymerase chain reaction(RT-nPCR) to detect HEVRNA in feces. The feces were collected from sheep with positive anti-HEV antibodies in a sheep farm in Xinjiang Autonomous Region.
Results:
Six of 54 (11.11%) sheep were positive for HEV RNA. PCR amplification products were cloned, sequenced and analyzed. The homology among HEV of the 6 sheep HEV ORF2 189bp nucleotide amplification sequences was 99.38%-100%. They should belong to the same genotype. They were respectively compared with HEV genotype I, II, III and IV corresponding 189bp nucleotide sequences. The average homology was 78.67%-85.33%, 81.33%-82.67%, 78.67%-84.00% and 84.67%-95.36%. The maximum homology between 6 nucleotide amplification sequences and one sequence of genotype IV was 94.04%-95.36%. Based on sequence of the nucleic acid fragments, a phylogenetic tree was constructed. Six sheep HEV ORF2 189bp nucleotide amplification sequences in this study and bovine HEV, swine HEV and human HEV locate the same evolutionary vine and belong to genotype IV.
Conclusion:
The finding suggested that infection of HEV probably exists in the sheep group in Xinjiang Autonomous Region and the sheep may be a new animal host except swine in origins of HEV infection.
