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Updated: Jun 9, 2026
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
Published on: December 9, 2022
[Effect of polyethylene glycol as adjuvant on hepatitis B virus DNA vaccine in vitro]
Junpeng Wang1, Ligan Lu, Hongli Song
1Key Laboratory of Cellular and Molecular Immunology of College of Medicine; 2Institute of Immunology, Henan University, Kaifeng 475001, China. jpwangchina@henu.edu.cn
Objective:
To explore whether polyethylene glycol (PEG) as adjuvant could enhance the humoral and cellular immune responses on hepatitis B virus DNA vaccine.
Methods:
C57BL/6J mice were immunized with PEG and pVAX-S2 or alone pVAX-S2. After these mice were finally immunized for 14 days, the anti-HBs IgG, T cell proliferation, the expression of cytokines and CTL in vivo were detected.
Results:
Compared to mice immunized with alone pVAX-S2, PEG as adjuvant could increase the production of anti-HBs IgG and HBsAg specific T cell proliferation. In addition, the expression of IL-4, IFN-gamma in CD4+ T cells and IFN-gamma in CD8+ T cells was higher than control groups. The PEG/ pVAX-S2 groups could elicit significantly in vivo HBsAg specific CTL responses.
Conclusions:
PEG as adjuvant could enhance humoral and cellular immune responses, as well as in vivo CTL activity.
