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[Detection of bovine, goat, pig and chicken derived ingredients in animal products with universal PCR-microarray
Fengyun Shi1, Jiankun Miao, Liping Zhang
1Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Abstract:
We analyzed the sequence of vertebrate molecular marker genes, then we selected the mitochondrial DNA (mtDNA) 16S rRNA gene as marker gene. In order to detect four kinds of animal-derived ingredients, which including bovine, goat, pig and chicken. We utilized a pair of universal primers, designed four sets of species-specific microarray probes and two pairs of quality control probes. We optimized the PCR amplifications and hybridization conditions, therefore these four kinds of animal-derived ingredients could be rapid and accurate detected by this approach. The detection limits were all reaches 1 pg. We established the detection platform of these four kinds of animal-derived ingredients. This universal PCR-microarray assay provides a new method for the identification of animal-derived ingredients in the import-export field.
