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Updated: Jun 9, 2026
High Pressure Single Crystal Diffraction at PX^2
Published on: January 16, 2017
Design of a standalone-type beryllium vessel for high-pressure protein crystallography
Yoshihisa Suzuki1, Masayuki Tsukamoto, Haruhiko Sakuraba
1Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan. suzuki@chem.tokushima-u.ac.jp
Abstract:
A standalone-type beryllium (Be) high-pressure crystallography vessel has been developed. Using a coupler-joint unit and a pressure valve, we could keep the pressure in the vessel constant at 100+/-1 MPa for more than 24 h without connecting to a pressure-generating apparatus. Diffraction spots of a glucose isomerase (GI) crystal under 100 MPa were collected using the vessel and a rotating copper-anode in-house x-ray generator (0.8 kW). We successfully collected a 2.0 A resolution data set of a 0.5 mm size GI crystal in an aqueous solution at 100 MPa.
