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Updated: Jun 9, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Urinary proteomics evaluation in interstitial cystitis/painful bladder syndrome: a pilot study.
Young Ah Goo1, Yihsuan S Tsai, Alvin Y Liu
1Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA.
Summary
This study identified distinct urinary protein profiles in women with interstitial cystitis/painful bladder syndrome (IC/PBS), revealing potential biomarkers and pathways involved in this chronic condition.
Area of Science:
- Urology
- Proteomics
- Biochemistry
Background:
- Interstitial cystitis/painful bladder syndrome (IC/PBS) is a chronic condition causing pelvic pain and discomfort.
- Understanding the molecular underpinnings of IC/PBS is crucial for developing diagnostic and therapeutic strategies.
Purpose of the Study:
- To profile the urinary proteome of women with IC/PBS using shotgun proteomics.
- To identify specific proteins and biological networks associated with IC/PBS.
Main Methods:
- Urine samples from 10 female IC/PBS patients and 10 healthy controls were analyzed.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed for protein identification.
- Gas-phase fractionation (GPF) enhanced protein identification, and peptide spectral counting quantified protein differences.
Main Results:
- Elevated levels of alpha-1B-glycoprotein (A1BG) and orosomucoid-1 (ORM1) were observed in IC/PBS patients compared to controls.
- Decreased levels of transthyretin (TTR) and hemopexin (HPX) were found in IC/PBS patients.
- Functional analysis indicated dysregulation of cell adhesion, response to stimuli, inflammation, wounding, and DNA damage repair pathways in IC/PBS.
Conclusions:
- Significant qualitative and quantitative differences exist in the urinary proteomes of women with and without IC/PBS.
- Specific proteins and pathways identified may contribute to IC/PBS pathology or result from the condition.
- These findings offer potential biomarkers for IC/PBS diagnosis and targets for future research.
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