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Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...

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Related Experiment Video

Updated: Jun 9, 2026

Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model
09:57

Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model

Published on: June 4, 2017

Directing stem cell trafficking via GPS.

Robert Sackstein1

  • 1Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Methods in Enzymology
|September 7, 2010
PubMed
Summary
This summary is machine-generated.

Engineered stem cells can now target tissue injury sites. Glycan engineering creates a specific cell surface marker, improving delivery for regenerative therapies.

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Last Updated: Jun 9, 2026

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High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
09:19

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

Published on: January 4, 2015

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Regenerative Medicine

Background:

  • Effective stem-cell therapy requires precise delivery of stem/progenitor cells to injured tissues.
  • Circulating cells must adhere strongly to endothelium to resist blood flow forces.
  • Selectins and their sialofucosylated glycan ligands mediate crucial cell-adhesion interactions.

Purpose of the Study:

  • To develop a method for enhancing stem/progenitor cell homing to sites of tissue injury.
  • To engineer cell surface glycans to improve intravascular delivery of stem cells.
  • To enable targeted delivery of regenerative therapeutics.

Main Methods:

  • Utilized glycosyltransferase-programmed stereosubstitution (GPS) for glycan engineering.
  • Modified CD44 glycans on living cells to create the hematopoietic cell E-/L-selectin ligand (HCELL).
  • Ex vivo engineering of cell surface glycans.

Main Results:

  • Successfully created HCELL on the surface of cells lacking it natively.
  • Demonstrated the ability to engineer cell surface glycans for targeted cell trafficking.
  • Enabled efficient vascular delivery of engineered stem/progenitor cells.

Conclusions:

  • Glycan engineering via GPS can create HCELL on various cell types.
  • This method facilitates targeted delivery of stem/progenitor cells to E-selectin expressing endothelium.
  • Ex vivo glycan modification of HCELL offers a promising strategy for regenerative medicine.