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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

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Published on: June 28, 2024

A versatile technology for cellular glycomics using lectin microarray.

Hiroaki Tateno¹, Atsushi Kuno, Yoko Itakura

¹Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central 2, Umezono, Ibaraki, Japan.

Methods in Enzymology

|September 7, 2010

Summary

Profiling the cellular glycome using lectin microarray allows for detailed cell discrimination. This approach, demonstrated with Chinese hamster ovary cells and mutants, advances the field of Cellular Glycomics.

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Area of Science:

Glycoscience
Cell Biology
Biochemistry

Background:

Cells possess a complex glycan layer, termed the cellular glycome, which acts as a cell-specific identifier.
The cellular glycome reflects cellular state and type, enabling detailed cell discrimination.
Understanding the cellular glycome is crucial for various biological and medical applications.

Purpose of the Study:

To develop and validate a strategy for profiling the cellular glycome.
To demonstrate lectin-based cell discrimination using the developed profiling method.
To explore the application of Cellular Glycomics in distinguishing cell types.

Main Methods:

Utilized lectin microarray technology for comprehensive glycan profiling of cells.
Employed Chinese hamster ovary (CHO) cells and their glycosylation-defective mutants (Lec1, Lec2, Lec8) as model systems.
Applied lectin-based approaches for discriminating between different cell types based on their glycome.

Main Results:

Successfully profiled the cellular glycome of model cell lines.
Demonstrated effective discrimination of CHO cells and their mutants based on glycan profiles.
Established a link between specific glycan patterns and cell type identity.

Conclusions:

Lectin microarray profiling is a powerful tool for characterizing the cellular glycome.
Cellular glycome analysis enables precise discrimination of cell types and states.
This work contributes to the advancement and practical applications of Cellular Glycomics.