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The Bombyx mori nucleopolyhedrovirus (BmNPV) ODV-E56 envelope protein is also a per os infectivity factor

Xingwei Xiang1, Lin Chen, Aiqin Guo

  • 1College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, PR China.

Virus Research
|September 7, 2010
PubMed

Insights

The Bombyx mori nucleopolyhedrovirus ODV-E56 envelope protein is crucial for per os infection in silkworm larvae. While essential for oral infection, it is not required for viral replication within the insect.

Area of Science:

  • Virology
  • Insect Pathology
  • Molecular Biology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen of silkworms.
  • The odv-e56 gene encodes the ODV-E56 envelope protein, a late viral gene product.

Purpose of the Study:

  • To elucidate the role of the ODV-E56 envelope protein in the BmNPV life cycle.
  • To investigate the necessity of ODV-E56 for viral replication and infectivity.

Main Methods:

  • Construction of an odv-e56 null virus (BmE56D) and a repaired virus (BmE56DR) via homologous recombination.
  • Analysis of budded virion (BV) and polyhedra production, viral DNA replication, and cell morphology.
  • Larval bioassays using per os inoculation and hemocoel injection.

Main Results:

  • No significant differences in BV/polyhedra production, viral DNA replication, or cell morphology between BmE56D, WT, and BmE56DR viruses.
  • BmE56D BV efficiently killed larvae upon injection into the hemocoel.
  • BmE56D failed to infect larvae when administered per os.

Conclusions:

  • The ODV-E56 envelope protein is a per os infectivity factor (PIF) for BmNPV.
  • ODV-E56 is not essential for viral replication within Bombyx mori cells.
  • This protein plays a critical role in the oral route of BmNPV infection.

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