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Updated: Jun 9, 2026

09:57
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
Improved method for bacterial cell capture after flow cytometry cell sorting
D Guillebault1, M Laghdass, P Catala
1UPMC Université Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France.
Applied and Environmental Microbiology
|September 7, 2010
Abstract:
Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.

