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Related Experiment Video

Updated: Jun 9, 2026

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

Near-infrared fluorescent proteins.

Dmitry Shcherbo¹, Irina I Shemiakina, Anastasiya V Ryabova

¹Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.

|September 7, 2010

Summary

New near-infrared fluorescent proteins, eqFP650 and eqFP670, offer enhanced brightness and red-shifted emission. These proteins are valuable tools for advanced whole-body imaging applications.

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Published on: January 5, 2015

Area of Science:

Biotechnology and Biomedical Imaging
Protein Engineering and Fluorescence Spectroscopy

Background:

Whole-body imaging techniques require fluorescent proteins emitting in the near-infrared (NIR) and infrared (IR) spectrum.
Existing NIR fluorescent proteins often lack sufficient brightness or optimal spectral properties for demanding applications.

Purpose of the Study:

To develop and characterize novel dimeric fluorescent proteins emitting in the near-infrared range.
To evaluate the brightness, spectral properties, and photostability of the newly engineered proteins for biological imaging.

Main Methods:

Engineering and characterization of dimeric fluorescent proteins.
Spectroscopic analysis of emission wavelengths, quantum yields, and extinction coefficients.
Assessment of photostability under prolonged illumination.

Main Results:

Reported development of near-infrared dimeric fluorescent proteins eqFP650 and eqFP670.
eqFP650 identified as the brightest fluorescent protein with emission maximum above 635 nm.
eqFP670 exhibits the most red-shifted emission maximum and high photostability among characterized proteins.

Conclusions:

The novel eqFP650 and eqFP670 proteins represent significant advancements in NIR fluorescent protein technology.
These proteins are highly suitable for advanced whole-body imaging, offering improved signal-to-noise ratios and deeper tissue penetration.
The enhanced spectral properties and photostability of these proteins expand the toolkit for in vivo biological research.