The exon junction complex differentially marks spliced junctions
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Updated: Jun 9, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Jérôme Saulière1, Nazmul Haque, Scot Harms
1Institut de Biologie de l'Ecole Normale Supérieure, Centre National de la Recherche Scientifique/ UMR 8197, Paris, France.
Abstract:
The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.
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