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RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...

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Related Experiment Video

Updated: Jun 9, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

The exon junction complex differentially marks spliced junctions.

Jérôme Saulière1, Nazmul Haque, Scot Harms

  • 1Institut de Biologie de l'Ecole Normale Supérieure, Centre National de la Recherche Scientifique/ UMR 8197, Paris, France.

Nature Structural & Molecular Biology
|September 7, 2010
PubMed
Summary
This summary is machine-generated.

The exon junction complex (EJC) deposition onto mRNAs is not automatic after splicing. Specific introns and RNA sequences regulate EJC

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Last Updated: Jun 9, 2026

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Genetics

Background:

  • The exon junction complex (EJC) is crucial for post-transcriptional RNA regulation in multicellular organisms.
  • EJC is deposited on messenger RNA (mRNA) during the splicing process.

Purpose of the Study:

  • To investigate the regulation of EJC deposition during mRNA processing.
  • To determine if EJC deposition is a constitutive or regulated event.
  • To identify factors influencing EJC association with specific mRNA junctions.

Main Methods:

  • Utilized Drosophila melanogaster cell models.
  • Analyzed EJC-dependent nonsense-mediated mRNA decay (NMD).
  • Investigated RNA cis-acting sequences influencing EJC binding.

Main Results:

  • Demonstrated that only a subset of introns triggers EJC-dependent NMD.
  • Showed that EJC association with spliced junctions is dependent on specific RNA cis-acting sequences.
  • Provided evidence that EJC deposition is not constitutive.

Conclusions:

  • EJC deposition is a regulated process, not a default outcome of splicing.
  • RNA cis-acting elements play a critical role in controlling EJC recruitment to specific mRNA sites.
  • This regulation impacts downstream post-transcriptional events like NMD.