Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fungal keratitis caused by a dematiaceous Pleosporales member following ocular trauma with a wooden fragment: a case report.

Journal of ophthalmic inflammation and infection·2026

Same author

Association between fungal growth temperature and corneal lesions in fungal keratitis: a case series.

BMC ophthalmology·2026

Same author

Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells.

The Journal of antibiotics·2026

Same author

Rhizopus arrhizus in a Thrombus Retrieved from Internal Carotid Artery Occlusion in Rhino-Orbito-Cerebral Mucormycosis: A Case Report.

Internal medicine (Tokyo, Japan)·2026

Same author

Late-onset <i>Exophiala dermatitidis</i> keratitis after cataract surgery: A case report and literature review.

Medical mycology case reports·2026

Same author

Rapidly Progressive Phaeohyphomycosis Caused by Pleurostoma richardsiae in an Immunosuppressed Patient.

The Journal of dermatology·2026

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

A rapid method for identifying Byssochlamys and Hamigera.

Motokazu Nakayama¹, Kouichi Hosoya, Tetsuhiro Matsuzawa

¹Global R&D Safety Science, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-Gun, Tochigi 321-3497, Japan.

Journal of Food Protection

|September 8, 2010

Summary

New PCR primers accurately identify heat-resistant fungi like Byssochlamys and Hamigera, crucial for preventing spoilage in heat-processed acidic foods. This method offers high specificity for food safety applications.

More Related Videos

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Published on: July 14, 2023

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Published on: April 21, 2012

Related Experiment Videos

Last Updated: Jun 9, 2026

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Dissection and Grading of Ovarian Development in Wild-Type Female Insects

Published on: July 14, 2023

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Published on: April 21, 2012

Area of Science:

Food Microbiology
Mycology
Molecular Biology

Background:

Heat-resistant fungi, including Byssochlamys, Talaromyces, Neosartorya, and Hamigera, cause spoilage in heat-processed acidic foods.
Ascospore formation by these fungi contributes to their resistance to heat processing.

Purpose of the Study:

To evaluate beta-tubulin gene differences between Byssochlamys and Hamigera.
To develop specific primers for identifying Byssochlamys species (fulva, nivea, spectabilis) and Hamigera.
To assess the specificity and detection limits of the developed primer sets.

Main Methods:

Comparative analysis of beta-tubulin gene sequences.
Development and application of specific Polymerase Chain Reaction (PCR) primers.
Testing primer specificity against various fungal species and DNA contamination levels.
Determining DNA detection limits using standard and nested PCR.

Main Results:

Specific PCR primers were successfully developed for Byssochlamys fulva, B. nivea, B. spectabilis, and Hamigera.
Primers B1F/1R detected B. fulva, B. nivea, B. langunculariae, and B. zollerniae.
Primers Pae4F/4R-1 and H2F/2R specifically identified B. spectabilis and Hamigera, respectively.
The developed primer sets demonstrated high specificity, even with 1,000-fold fungal DNA contamination.
Detection limits were as low as 1 ng DNA (PCR) and 10 pg DNA (nested PCR).

Conclusions:

The developed primer sets provide a highly specific method for identifying key heat-resistant fungal species.
This molecular tool can aid in monitoring and controlling fungal contamination in food processing.
The high specificity ensures reliable detection, crucial for ensuring food safety and quality.