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Accelerators in concrete serve as admixtures to speed up the hardening process, enabling the concrete to achieve early strength faster. Although accelerators do not necessarily impact the time it takes concrete to set, they reduce this time in practice. A common accelerator is calcium chloride, which is particularly useful for hastening early strength development in cold weather or for rapid repair jobs that require quick heat generation after mixing.
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Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
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Improved sample and reagent pipetting with the MINILAB.

I Lindahl1, P Masson

  • 1Dept. of Clinical Chemistry, University Hospital Lund, Sweden.

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

The MINILAB photometer showed poor precision for cholesterol and bilirubin tests. Using a Microcap for pipetting significantly improved accuracy, making it suitable for clinical use.

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Area of Science:

  • Clinical chemistry
  • Analytical instrumentation

Background:

  • The MINILAB is a compact photometer for small laboratories and physician offices.
  • It offers preprogrammed methods for various analytes in blood, plasma, or serum.
  • Kits include disposable cuvettes and capillaries for sample and reagent handling.

Purpose of the Study:

  • To evaluate the precision of the MINILAB photometer for serum cholesterol and bilirubin determination.
  • To identify potential sources of error in the assay methodology.
  • To assess the impact of a modified pipetting technique on assay precision.

Main Methods:

  • Assessed MINILAB precision for serum cholesterol and bilirubin using manufacturer-recommended protocols.
  • Investigated sample and reagent pipetting as a potential source of variability.
  • Implemented a modified pipetting technique using a Microcap for improved accuracy.

Main Results:

  • Initial precision unacceptable, with coefficients of variation (CV) up to 16% for cholesterol and 43% for bilirubin.
  • Modification using Microcap pipetting drastically improved precision.
  • Achieved CVs of 1.4% for cholesterol and 3.5% for bilirubin after modification.

Conclusions:

  • The standard MINILAB assay protocol exhibits significant imprecision for critical analytes.
  • Microcap-assisted pipetting is a simple and effective method to enhance MINILAB assay precision.
  • This modification makes the MINILAB a more reliable diagnostic tool for clinical settings.