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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Light Acquisition02:16

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

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Related Experiment Video

Updated: Jun 9, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Talbot array illuminator with multilevel phase gratings.

P Szwaykowski, V Arrizon

    Applied Optics
    |September 8, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed one-dimensional multilevel phase gratings that create amplitude binary gratings with adjustable opening ratios. These gratings function as array illuminators, offering high compression and numerous uniform illumination points.

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    Last Updated: Jun 9, 2026

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

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    Published on: January 28, 2019

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    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    Area of Science:

    • Optics and Photonics
    • Diffractive Optics

    Background:

    • Phase gratings are crucial optical elements.
    • Controlling light intensity distribution is a key challenge in optics.

    Purpose of the Study:

    • To construct novel one-dimensional multilevel phase gratings.
    • To achieve arbitrary control over amplitude binary grating parameters.
    • To develop efficient array illuminators.

    Main Methods:

    • Fabrication of one-dimensional multilevel phase gratings.
    • Illumination with coherent plane waves.
    • Analysis of resulting amplitude binary gratings.

    Main Results:

    • Formation of amplitude binary gratings with arbitrary opening ratios.
    • Demonstration of gratings as array illuminators.
    • Achieved high compression factors and numerous uniformly illuminated points.

    Conclusions:

    • The proposed phase gratings offer precise control over light modulation.
    • These gratings are effective as array illuminators for various applications.
    • Significant advancements in diffractive optical element design were achieved.