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Phase Contrast and Differential Interference Contrast Microscopy01:26

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 9, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Inexpensive range camera operating at video speed.

J Kramer, P Seitz, H Baltes

    Applied Optics
    |September 8, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study presents a novel optoelectronic device for real-time 3D object surface mapping using active triangulation. The system generates 50 depth maps per second, encoded in video signals for enhanced visualization.

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    Last Updated: Jun 9, 2026

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    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    Area of Science:

    • Optoelectronics
    • Computer Vision
    • 3D Sensing

    Background:

    • Real-time 3D surface data acquisition is crucial for various applications.
    • Existing methods often face limitations in speed and processing.
    • Active triangulation offers a viable approach for depth recovery.

    Purpose of the Study:

    • To develop and demonstrate an optoelectronic device for real-time acquisition and display of object surface range data.
    • To achieve high-speed depth map generation using active triangulation.
    • To encode depth information into standard video signals for practical use.

    Main Methods:

    • Utilized active triangulation with a galvanometer scanner to sweep a light sheet across the object.
    • Employed a specialized optical sensor and hardware for high-speed signal processing.
    • Generated 50 range maps per second, converting them into European standard video signals.

    Main Results:

    • The device acquires and displays object surface range data in real-time video.
    • Depth information is encoded in gray levels or color within the video signal.
    • Achieved an image resolution of 128 x 500 pixels with 1.5% depth accuracy.

    Conclusions:

    • The developed optoelectronic device successfully provides real-time 3D surface mapping.
    • The system's high frame rate and depth encoding offer practical advantages for 3D visualization.
    • The adaptable design allows for customization of measurement volume and dimensions.