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[Determination of two stilbene glycoside in five processed products of Rheum palamatum]
Li Li1, Cun Zhang, Yongqing Xiao
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. lili7755@163.com
Objective:
To compare of the contents of two stilbene glycoside in five processed products of Rheum palamatum.
Method:
The contents of trans-3, 5, 4'-trihydroxystilbene-4'-O-beta-D-glucopyranoside (a) and trans-3, 5, 4'-trihydroxystilbene-4'-Obeta-D-(6"-O-galloyl)-glucopyranoside (b) were determined by HPLC analysis at 35 degrees C with methanol-1% acetic acid as mobile phase, the wavelengths were set at 280, 300 nm, the flow rate was 1.0 mL x min(-1).
Result:
The two components could be detected in five processed products, and their contents were more close in the no-parched pieces, the vinegar roasts pieces and the wine roast pieces. However, the contents reduced significantly in other two kinds of pieces which were lower than 80 percent of no-parched pieces.
Conclusion:
High temperature may result in a significant reduction on glycoside in the pieces of rhubarb, and we have received similar results from determination of other glycoside compounds. Further analysis and comparison with the content of their corresponding aglycones, can provide a scientific basis to explain the variation of the material basis in the processed products of rhubarb.
