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[Protective effects of asiaticoside on sepsis-induced acute kidney injury in mice]
Jiajia Zheng1, Li'na Zhang, Mengjiao Wu
1Chongqing Key of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China.
Objective:
The present study is to investigate the protective effects of asiaticoside on sepsis-induced acute kidney injury in mice.
Method:
With the sepsis induced by cecal ligation and puncture (CLP), forty eight kunming mice were randomly divided into four groups as sham operated, CLP treated, CLP + asiaticoside 15, 45 mg x kg(-1) groups. General conditions and the amount of dead rate of mice were observed. The BUN and Cr levels were observed by the kits. IL-6 in serum was assayed by enzyme-linked immunosorbent assay (ELISA). Kidney tissues were harvested for determination of iNOS expression by Western blotting analysis. The pathologic changes were observed under electron microscope via hematoxylin-eosin (HE) stain.
Result:
Compared with CLP group, the death rate, the levels of BUN, Cr, IL-6, and iNOS protein expression of asiaticoside groups were significantly reduced. The pathologic changes in kidney tissues induced by sepsis were significantly attenuated dose-dependently by asiaticoside under electron microscope.
Conclusion:
Asiaticoside has protective effects against sepsis-induced acute kidney injury, which were probably associated with the inhibition of IL-6 in serum and iNOS protein in kidney tissues.
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