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Ferric Chloride-induced Murine Thrombosis Models
Published on: September 5, 2016
Silver nanoparticles enhance thrombus formation through increased platelet aggregation and procoagulant activity
Eun-Ah Jun1, Kyung-Min Lim, Keunyoung Kim
1College of Pharmacy, Seoul National University, Shinrim-dong San 56-1, Gwanak-ku, Seoul, Korea.
Nanotoxicology
|September 9, 2010
Summary
Silver nanoparticles (nano Ag) increase platelet activation and blood clot formation, contributing to thrombotic diseases. This occurs through calcium signaling and potentiates effects in already activated platelets.
Area of Science:
- Biomedical Science
- Nanotechnology
- Toxicology
Background:
- Silver nanoparticles (nano Ag) are widely used, but their toxicity is not fully understood.
- Platelet aggregation and procoagulant activation are critical factors in thrombotic diseases.
Purpose of the Study:
- To investigate the effects of nano Ag on human platelet activation and procoagulant activity.
- To determine the in vivo impact of nano Ag exposure on thrombus formation and platelet function.
Main Methods:
- In vitro studies using freshly isolated human platelets to assess aggregation, phosphatidylserine exposure, and thrombin generation.
- In vivo studies in rats involving intravenous or intratracheal administration of nano Ag, followed by ex vivo analysis of thrombus formation and platelet parameters.
Main Results:
- Nano Ag induced platelet aggregation, phosphatidylserine exposure, and thrombin generation in vitro.
- Sub-threshold thrombin levels significantly enhanced nano Ag-induced platelet activation.
- Nano Ag increased intracellular calcium, P-selectin expression, and serotonin release.
- In vivo, nano Ag exposure enhanced venous thrombus formation, platelet aggregation, and phosphatidylserine externalization.
Conclusions:
- Nano Ag promotes platelet activation and procoagulant activity, potentially increasing the risk of thrombotic events.
- The prothrombotic effects of nano Ag are amplified in the presence of existing platelet activation.
- These findings highlight the potential thrombotic risks associated with silver nanoparticle exposure.
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