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Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA
Pavel Neuzil1, Fang Cheng, Jeffrey Bo Woon Soon
1Institute of Microelectronics, 11 Science Park Road, Singapore. pavel@kist-europe.de
Lab on a Chip
|September 9, 2010
Abstract:
This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.
