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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 9, 2026

Examination of Pyroptosis by Flow Cytometry
05:14

Examination of Pyroptosis by Flow Cytometry

Published on: May 31, 2024

Flow cytometry in veterinary oncology.

F Reggeti1, D Bienzle

  • 1Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada.

Veterinary Pathology
|September 10, 2010
PubMed
Summary

Flow cytometry offers precise cell analysis for veterinary oncology, aiding in diagnosing round cell tumors and assessing tumor proliferation. This technology enhances patient management through detailed immunophenotyping and DNA content analysis.

Area of Science:

  • Veterinary Medicine
  • Veterinary Oncology
  • Cell Biology

Background:

  • Flow cytometry is a sensitive technique for multi-parameter single-cell analysis.
  • Its application in veterinary oncology is expanding due to new species-specific antibodies.
  • Key applications include immunophenotyping and DNA content analysis.

Purpose of the Study:

  • To review the technical aspects of flow cytometry for veterinary applications.
  • To discuss the availability of suitable antibodies for domestic animal studies.
  • To highlight flow cytometry's role in veterinary oncology, particularly for round cell tumors.

Main Methods:

  • Utilizing fluorescently labeled antibodies for immunophenotyping.
  • Employing fluorescent dyes for DNA content analysis to determine cell proliferation.

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Last Updated: Jun 9, 2026

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  • Characterizing cell markers and DNA ploidy in animal tumor samples.

    • Main Results:

    • Flow cytometry aids in classifying round cell tumors, including lymphocyte proliferations, with potential benefits for patient management.
    • DNA content analysis shows promise for assessing tumor proliferation as an adjunct to traditional classification.
    • The diagnostic and prognostic value is still under investigation but shows positive trends.

    Conclusions:

    • Flow cytometry is a valuable tool in veterinary oncology for immunophenotyping and DNA analysis.
    • Further standardization and validation are needed for DNA analysis in routine veterinary oncology.
    • This technique holds promise for improving the diagnosis and management of animal cancers.