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Related Experiment Video

Updated: Jun 9, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

MicroRNA-profiling in formalin-fixed paraffin-embedded specimens.

Ulrich Lehmann1

  • 1Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany. Lehmann.Ulrich@MH-Hannover.de

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2010
PubMed
Summary

This study details RNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues for microRNA profiling. It enables microRNA analysis in valuable FFPE samples using bead technology.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Small regulatory RNAs, particularly microRNAs, significantly impact biological and pathological processes.
  • MicroRNA expression analysis is a crucial tool in modern biology and pathology.
  • Formalin-fixed paraffin-embedded (FFPE) tissues are a valuable but challenging source for molecular analysis.

Purpose of the Study:

  • To describe a method for extracting RNA from FFPE samples.
  • To present a protocol for microRNA profiling of extracted RNA using bead technology.
  • To enable the analysis of microRNAs in precious FFPE specimens.

Main Methods:

  • RNA extraction from FFPE tissue samples.
  • MicroRNA profiling using fluorescence-labeled bead technology (Luminex Inc.).

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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Last Updated: Jun 9, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Main Results:

  • Successfully extracted RNA suitable for microRNA profiling from FFPE samples.
  • Demonstrated the feasibility of microRNA profiling on FFPE-derived RNA using bead-based assays.

Conclusions:

  • RNA extraction and microRNA profiling from FFPE samples are achievable.
  • This methodology expands the utility of FFPE archives for microRNA research.
  • Facilitates the study of microRNA expression in diverse pathological contexts.