Related Experiment Video
Updated: Jun 9, 2026

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
Published on: September 8, 2021
Three-dimensional structures of DNA-bound transcriptional regulators
Tripti Shrivastava1, Tahir H Tahirov
1Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA. tshrivastava@unmc.edu
Abstract:
Our understanding of the detailed mechanisms of specific promoter/enhancer DNA-binding site recognition by transcriptional regulatory factors is primarily based on three-dimensional structural studies using the methods of X-ray crystallography and NMR. Vast amount of accumulated experimental data have revealed the basic principles of protein-DNA complex formation paving the way for better modeling and prediction of DNA-binding properties of transcription factors. In this review, our intent is to provide a general overview of the three-dimensional structures of DNA-bound transcriptional regulators starting from the basic principles of specific DNA recognition and ending with high-order multiprotein-DNA complexes.
Related Concept Videos
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Co-activators and Co-repressors
Co-activators and Co-repressors
Cis-regulatory Sequences
Cis-regulatory Sequences

