Synthesis of a 2-selenothymidine phosphoramidite and its incorporation into oligodeoxyribonucleotides
1Georgia State University, Atlanta, Georgia, USA.
Current Protocols in Nucleic Acid Chemistry
|September 10, 2010
Abstract:
The detailed synthetic protocol for a 2-selenothymidine phosphoramidite and its use in preparing Se-derivatized oligonucleotides are described here. The Se-modified phosphoramidite synthesis was achieved by activating a 2-thiothymidine derivative, followed by introduction of selenium functionality. The coupling reaction yield of the 2-selenothymidine phosphoramidite during solid-phase synthesis is high (>95%), and the oligonucleotides containing the 2-selenothymidine derivatization are stable.
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