Related Experiment Video
Updated: Jun 9, 2026

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
Published on: September 9, 2020
A reliable method to concentrate circulating DNA
Olga Bryzgunova1, Anna Bondar, Evgeniy Morozkin
1Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia. olga.bryzgunova@niboch.nsc.ru
Abstract:
Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipitation of circulating DNA with acetone in the presence of triethylamine provides minimal DNA loss, high reproducibility, and at least three times higher DNA yield in comparison with the standard ethanol protocol.
Related Concept Videos
DNA Isolation
DNA Isolation

