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Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Jun 9, 2026

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

A reliable method to concentrate circulating DNA.

Olga Bryzgunova¹, Anna Bondar, Evgeniy Morozkin

¹Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia. olga.bryzgunova@niboch.nsc.ru

Analytical Biochemistry

|September 11, 2010

Summary

This summary is machine-generated.

Concentrating cell-free DNA (cfDNA) is crucial for molecular studies. Acetone precipitation offers a superior method, yielding significantly more DNA with minimal loss compared to standard ethanol protocols.

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Last Updated: Jun 9, 2026

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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Circulating DNA concentration is essential for downstream molecular analyses like PCR, sequencing, and microarrays.
Accurate quantification and amplification of cfDNA are critical for diagnostic and research applications.

Purpose of the Study:

To compare the efficacy of five distinct methods for concentrating circulating DNA from blood samples.
To identify an optimal protocol for maximizing cfDNA yield and reproducibility.

Main Methods:

Evaluated five different protocols for cfDNA concentration.
Included precipitation with acetone/triethylamine and standard ethanol precipitation.
Assessed DNA yield and reproducibility for each method.

Main Results:

Acetone precipitation in the presence of triethylamine demonstrated superior performance.
This method resulted in minimal DNA loss and high reproducibility.
Achieved at least a threefold increase in DNA yield compared to the standard ethanol protocol.

Conclusions:

Acetone precipitation with triethylamine is a highly effective method for concentrating circulating DNA.
This protocol significantly enhances DNA yield, making it ideal for sensitive molecular studies.
Offers a substantial improvement over traditional ethanol-based methods for cfDNA isolation.