Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How to Foster Challenging Interdisciplinary Collaborations: Can Philosophy Support Neuroscientists?

The European journal of neuroscience·2026

Same author

Role of heparin-induced HGF release in the acute phase of STEMI.

Frontiers in cardiovascular medicine·2025

Same author

A re-innervated <i>in vitro</i> skin model of non-histaminergic itch and skin neurogenic inflammation: PAR2-, TRPV1- and TRPA1-agonist induced functionality.

Skin health and disease·2022

Same author

WITHDRAWN: Distribution of the Novel RFRP-3/receptors system in the epididymis of the seasonal desert rodent, Gerbillus tarabuli, during sexual activity.

Morphologie : bulletin de l'Association des anatomistes·2021

Same author

Real-world implementation of a geriatric-specific ERAS protocol in patients undergoing colonic cancer surgery.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2020

Same author

G protein-coupled oestrogen receptor 1, oestrogen receptors and androgen receptor in the sand rat (Psammomys obesus) efferent ducts.

Folia morphologica·2020

Same journal

Single-cell transcriptomic profiling identifies TIMP1 as a diagnostic marker linked to macrophage ferroptosis in ulcerative colitis.

Tissue & cell·2026

Same journal

From lab to life: Advances in tissue engineering and 3D bioprinting for regenerative female reproductive health.

Tissue & cell·2026

Same journal

Morphological and ultrastructural characterization of the male accessory glands in Moechotypa diphysis (Coleoptera: Cerambycidae).

Tissue & cell·2026

Same journal

Annexin A1 regulates intestinal epithelial homeostasis and NLRP3-associated signaling in experimental type 1 diabetes.

Tissue & cell·2026

Same journal

CD38-driven NAD⁺ depletion governs crypt structure and barrier dysfunction in fluorouracil-induced colonic mucositis: From metabolic collapse to architectural remodeling.

Tissue & cell·2026

Same journal

Dual regulatory roles and mechanisms of Gamma delta T cells in organ fibrosis.

Tissue & cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Calpains expression during Xenopus laevis development.

E N Moudilou¹, N Mouterfi, J-M Exbrayat

¹Université de Lyon, UMRS 449, Laboratoire de Biologie Générale, Université Catholique de Lyon, Reproduction et développement comparés, EPHE, 25 Rue du Plat, F-69288 Lyon Cedex 02, France.

|September 11, 2010

Summary

This summary is machine-generated.

Calpains (calcium-activated proteases) are crucial for Xenopus development. Their protein expression peaks during organogenesis and tissue remodeling in tadpoles, suggesting a key role in developmental processes.

More Related Videos

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Related Experiment Videos

Last Updated: Jun 9, 2026

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

Area of Science:

Developmental Biology
Molecular Biology
Proteomics

Background:

Calpains are calcium-activated cytoplasmic proteases.
Calpains are involved in cell differentiation and apoptosis.
Calpains 1-3 are the most studied calpain enzymes.

Purpose of the Study:

To determine the protein localization of calpains 1-3 during Xenopus laevis development.
To elucidate the functional roles of calpains 1-3 in developmental processes.

Main Methods:

Immunohistochemistry was used to detect calpain protein localization.
Analysis spanned from the one-cell stage to adult Xenopus laevis.

Main Results:

Calpain 1-3 protein expression was detected throughout Xenopus development.
Expression levels were low in early stages, increased significantly in tadpoles, and decreased during metamorphosis and adulthood.
Maximal calpain expression coincided with organogenesis and tissue modeling.

Conclusions:

Calpains 1-3 exhibit dynamic expression patterns during Xenopus development.
These findings indicate a critical role for calpains in developmental processes, particularly organ formation and tissue remodeling.