Related Concept Videos
01:05Total Internal Reflection Fluorescence Microscopy
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
01:16Focusing of Light in the Eye
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
02:16Light Acquisition
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
01:26Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
01:16Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Updated: Jun 8, 2026
10:35
Bringing the Visible Universe into Focus with Robo-AO
Published on: February 12, 2013
Stray-light suppression in a reflecting white-light coronagraph.
Applied Optics
|September 11, 2010
Summary
This study analyzes stray-light suppression in the Solar and Heliospheric Observatory's Ultraviolet Coronagraph Spectrometer. The design effectively minimizes stray light, ensuring high-quality solar observations.
Area of Science:
- Solar physics
- Space instrumentation
- Optical engineering
Background:
- The Solar and Heliospheric Observatory (SOHO) mission requires precise measurements of the solar corona.
- Stray light within coronagraph instruments can contaminate scientific data.
- The Ultraviolet Coronagraph Spectrometer (UVIS) white-light channel employs advanced optical designs for stray-light mitigation.
Purpose of the Study:
- To analyze the stray-light suppression performance of the UVIS white-light channel.
- To quantify contributions from various stray-light sources.
- To validate the instrument's design for effective stray-light rejection.
Main Methods:
- Laboratory testing of the white-light channel.
- Analytical modeling of stray-light propagation.
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Main Results:
- Stray-light contributions from direct sunlight and diffraction were quantified.
- Total stray-light levels were determined for heliocentric heights from 1.4 to 5 solar radii.
- Anticipated signal-to-stray-light ratios were calculated.
- The polarimeter section demonstrated effective stray-light rejection.
Conclusions:
- The UVIS white-light channel's stray-light suppression design is highly effective.
- The instrument is well-suited for detailed observations of the solar corona.
- The analysis confirms the instrument's capability to achieve high signal-to-stray-light ratios.