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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 8, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Integrated fiber optic probe for dynamic light scattering.

H S Dhadwal, R R Khan, K Suh

    Applied Optics
    |September 11, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study presents an integrated fiber optic probe for dynamic light scattering measurements. The novel probe enables accurate particle size determination with a ±1% accuracy for polystyrene latex spheres.

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    Last Updated: Jun 8, 2026

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    Scanning Light Scattering Profiler (SLPS) Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

    Published on: June 6, 2017

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Physical Chemistry

    Background:

    • Dynamic light scattering (DLS) is a technique used to determine the size of particles in suspension.
    • Accurate particle size measurement is crucial in various scientific and industrial applications.
    • Existing DLS systems can be complex and expensive, necessitating simpler and more cost-effective solutions.

    Purpose of the Study:

    • To develop and characterize an integrated fiber optic probe for coherent detection in dynamic light scattering.
    • To assess the accuracy and reliability of the developed probe for particle size analysis.

    Main Methods:

    • Fabrication of an integrated fiber optic probe by fusion splicing a monomode optical fiber with a graded-index multimode fiber.
    • Cleaving the multimode fiber to create a gradient-index fiber lens with specific focal length and f-number.
    • Utilizing the integrated fiber receiver to measure intensity-intensity autocorrelation functions of polystyrene latex sphere suspensions.
    • Analyzing 100 independent data sets to determine particle size recovery accuracy.

    Main Results:

    • The fabricated fiber optic probe functions effectively as a coherent receiver in DLS measurements.
    • Accurate particle size recovery (±1%) was achieved for an aqueous suspension of polystyrene latex spheres.
    • The integrated fiber lens design contributes to the probe's high performance.

    Conclusions:

    • The integrated fiber optic probe offers a promising and accurate solution for DLS measurements.
    • This technology can potentially simplify DLS instrumentation and improve accessibility for particle size analysis.
    • The ±1% accuracy demonstrates the potential of this probe for precise scientific investigations.