Related Experiment Video
Updated: Jun 8, 2026

Micro/Nano-scale Strain Distribution Measurement from Sampling Moiré Fringes
Published on: May 23, 2017
Interference microscopy and Fourier fringe analysis applied to measuring the spatial refractive-index distribution
Abstract:
We have applied the technique of Fourier fringe analysis to microscopic interferograms of needle crystals that grow from a solution. We use a differential technique in which an empty field interferogram is compared with one that contains distortion and obscuration by the growing crystal, and we demonstrate both analytically and experimentally a phase shift sensitivity of 0.01 fringe with a spatial resolution of half of a fringe spacing (~1 µm). Following the analysis of the interferogram in two dimensions, we show that the three-dimensional refractive-index field around the crystal can be deduced, assuming that it is axially symmetric, by an iterative method.
Related Concept Videos
Interference and Diffraction
IR Spectrometers
Total Internal Reflection Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

