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Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Tchern Lenn1, Christos N Gkekas, Laurent Bernard

  • 1Division of Biology, Imperial College London, London, UK SW7 2AZ.

Chemical Communications (Cambridge, England)
|September 11, 2010
PubMed

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...